Quantitative Determination of Key Components in Red Flower Oil by FT-IR Spectroscopy

 Introduction

This application note details a quantitative FTIR spectroscopy method for determining the key components in Red Flower Oil—methyl salicylate, eugenol, and α-pinene. Utilizing a PerkinElmer Spectrum 3 FTIR spectrometer with a ZnSe ATR accessory, the study developed PLS calibration models based on data from GC measurements. The method demonstrated high accuracy and precision, with SEP values under 1% for eugenol and α-pinene and under 2% for methyl salicylate. This approach offers a quick, reliable alternative to GC, enabling efficient quality control in accordance with pharmacopoeial standards.